Diversity Analysis of Cymbidium goeringii according to the Morphological Characteristics and Microsatellite DNA Locus
저자: Lee, Dae Gun (이대건)
소속: Department of Horticulture, The Graduate School, Catholic University of Daegu
지도교수: Professor Koh, Jae Chul
발행일: 2011년 08월
(Abstract)
Morphological characteristics of the flowers of Cymbidium goeringii were examined and analyzed. The tested samples revealed that the size of flowers and sepals of Chinese Cymbidium goeringii was larger than Korean ones. And Chinese Cymbidium goeringii showed 54% more anthocyanin spots than Korean ones in the upper part of column and between lip spotted and lip bump anthocyanin spots in Chinese Cymbidium goeringii appeared twice as much as Korean ones, while Korean Cymbidium goeringii showed more anthocyanin spots in peduncles, bracts, and lips than Chinese ones. In the length and width of lip spotted, Korean ones were larger. The shape of lip spotted in Chinese ones was more of V type, and that of Korean ones was more of U type.
Korean Cymbidium goeringii was examined and analyzed according to the places: eastern part and western part of Korea. Few anthocyanin spots were discovered between lip spotted and lip bump in the Cymbidium goeringii grown in the western part of Korea. However those grown in the eastern part showed 44% more small and light spots than those in the western part.
The red anthocyanin in bract of eastern Cymbidium goeringii was 30% deeper than in western ones. The vertical section of sepal of western Cymbidium goeringii bend inside slightly more than that of eastern ones, and the difference was 0.38. The red anthocyanin in sepal of eastern ones was lighter than that in western ones.
In this study S.S.R (simple sequence repeat) method was utilized to analyze and compare among the collections from three different regions; the eastern and western parts of Korea, and China. And GENEPOP S/W program was also used to see their similarities. The result shows the high similarity among them and the similarity range was 0.865~0.999. No differences in DNA was found among 11 markers between the eastern parts and the western parts in Korea.
According to the results of allele analysis, Chinese Cymbidium goeringii showed a lot more diversity than Korean ones. From the results it could be said that Korean Cymbidium goeringii was originated from China. The significant genetic differences among different markers in Korea and China would lead to the separation of Korean Cymbidium goeringii and Chinese ones. Furthermore, markers showing significant genetic differences can be utilized for identifying the origin of Cymbidium goeringii with genetic difference.
As mentioned previously, the genetic similarities in the allele analysis would suggest that the origin of Korean Cymbidium goeringii is China. Great differences between Korean Cymbidium goeringii and Chinese Cymbidium goeringii found in the present study suggest that the seeds can't be easily moved from China to Korea.